Fig. 7: Inhibitory effect of MSC-CM on Stat3 signaling and tumour growth in vivo. | Cell Death & Disease

Fig. 7: Inhibitory effect of MSC-CM on Stat3 signaling and tumour growth in vivo.

From: MSCs inhibit tumor progression and enhance radiosensitivity of breast cancer cells by down-regulating Stat3 signaling pathway

Fig. 7

(a) Renilla luciferase (Rluc) imaging of p-Stat3. Representative animals were injected with 2 × 106 MDA-MB-231 (Fluc/GFP-pStat3/Rluc) cells into the upper and lower right side of the mouse body after treatment with MSC-CM and control medium for 48 h. Quantitative analysis of Rluc signals indicated decreased p-Stat3 level. *P < 0.05 vs Ctrl, n = 6. (b) Firefly luciferase (Fluc) imaging of tumour growth. Representative animals injected with 2 × 106 MDA-MB-231 (Fluc/GFP-pStat3/Rluc) cells into the upper and lower right of the mouse body after treatment with MSC-CM and control medium for 48 h. Quantitative analysis of the Fluc signal. **P < 0.01 vs Ctrl, n = 6. (c) Renilla luciferase (Rluc) imaging of p-Stat3. Representative animals injected with 2 × 106 MDA-MB-231 (Fluc/GFP-pStat3/Rluc) cells into the upper and lower right side of the mouse body after treatment with MSC-CM combination with radiation or radiation alone. Quantitative analysis of Rluc signal. *P < 0.05 vs 4 Gy, n = 6. (d) Firefly luciferase (Fluc) imaging of tumour growth. Representative animals injected with 2 × 106 MDA-MB-231 (Fluc/GFP-pStat3/Rluc) cells into the upper and lower right side of the mouse body after treatment with MSC-CM combination with radiation or radiation alone. Quantitative analysis of Fluc signal. *P <  0.05 vs 4 Gy, n = 6

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