Fig. 4: NOX4 positively regulates the expression of EGFR. | Cell Death & Disease

Fig. 4: NOX4 positively regulates the expression of EGFR.

From: NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR

Fig. 4: NOX4 positively regulates the expression of EGFR.

MKN-45 and AGS cells were transfected with siRNA-NOX4 or siRNA-Negative Control for 24 h. a The expression of selected growth factor receptors was detected by immunoblotting with the indicated antibodies. b Relative mRNA expression levels were measured by RT-qPCR. *P < 0.05. c EGFR expression was detached by immunofluorescence (magnification ×500). d MKN-45 and AGS cells were transfected with siRNA-NOX4, siRNA-Negative Control, plasmid-NOX4 or plasmid-Negative Control for 24 h. Expression of NOX4 and EGFR was detected by immunoblotting with the indicated antibodies. e MKN-45 and AGS cells were cultured in adherent or suspended conditions for 24 h, expression of EGFR and p-EGFR was detected by immunoblotting with the indicated antibodies. f MKN-45 and AGS cells were transfected with siRNA-NOX4, siRNA-Negative Control, plasmid-NOX4 or plasmid-Negative Control for 24 h. Expression of EGFR and p-EGFR was detected by immunoblotting with the indicated antibodies. All experiments were performed in triplicate

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