Fig. 5: NOX4 regulates anoikis resistance and EGFR expression via ROS generation. | Cell Death & Disease

Fig. 5: NOX4 regulates anoikis resistance and EGFR expression via ROS generation.

From: NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR

Fig. 5

MKN-45 and AGS cells were treated with si-NC, NAC, si-NOX4, si-NOX4 + NAC or si-NOX4 + H2O2 (1 μM). (a) The expression of NOX4 and EGFR was detected by immunoblotting with the indicated antibodies. (b) The rate of anoikis in MKN-45 and AGS cells was measured by flow cytometry using Annexin V-FITC. *P < 0.05, **P < 0.01, ***P < 0.001. (c) Cellular morphology of MKN-45 and AGS cells in suspension conditions captured by microphotography (magnification x100). (d) The average aggregated number of MKN-45 and AGS suspension cells in each field was measured after 24 h of culturing. *P < 0.05, ***P < 0.001. All experiments were performed three independent times

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