Fig. 7: In vivo role of NOX4 in anoikis resistance and distant metastasis. | Cell Death & Disease

Fig. 7: In vivo role of NOX4 in anoikis resistance and distant metastasis.

From: NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR

Fig. 7

Data from tail vein injection model were shown in panels (a–g) and data from xenograft model were shown in panels (h–j). a Verifying of infection efficiency by fluorescence (magnification ×200). b Validation of knockdown efficiency of shRNA-NOX4 using western blot. c, d MKN-45-luciferase cells with or without NOX4 knockdown were tail vein injected into nude mice. c Bioluminescence imaging was performed with an IVIS Lumina XR at week 8. d Average luminescence intensity of each group at week 8. *P < 0.05. e Number of metastatic nodules in lungs of mice in group-shRNA-NOX4 and group-shRNA-NC. **P < 0.01. f Representative image of lung of mice in group-shRNA-NOX4 and group-shRNA-NC. Bars represent 5 mm for morphology and 200 μm for H&E staining. g Percentage of mice with metastasis in group-shRNA-NOX4 and group-shRNA-NC. h Image of tumors of mice in group-MKN45-AR, group-MKN45-AR + Plumbagin, group-MKN45 and group-MKN45 + Plumbagin. i Volume of tumor of mice in group-MKN45-AR, group-MKN45-AR + Plumbagin, group-MKN45 and group-MKN45 + Plumbagin at indicated time. j Weight of tumor of mice in group-MKN45-AR, group-MKN45-AR + Plumbagin, group-MKN45 and group-MKN45 + Plumbagin on 28th days. ***P < 0.001

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