Fig. 5: P36 upregulates p11 protein without affecting transcript levels. | Cell Death & Disease

Fig. 5: P36 upregulates p11 protein without affecting transcript levels.

From: Regulation of cell surface protease receptor S100A10 by retinoic acid therapy in acute promyelocytic leukemia (APL)☆

Fig. 5

Immunoblot analysis of (a) peritoneal macrophages isolated from p36+/+ and p36−/− mice treated for 24 h using 3 μM LC or 10 μM PYR-41 and (b) HEK293T cells transiently transfected using pcDNA3.1-empty vector or pcDNA3.1-p36 vector. Cell lysates were prepared and the levels of the indicated proteins were examined by immunoblot analysis with β-actin used as a loading control. Total RNA extracted from (c) HEK293T cells transiently transfected using pcDNA3.1-empty vector or pcDNA3.1-p36 vector were used for cDNA synthesis. The relative expression of p11 and p36 mRNA levels was determined from cDNA (25 ng) by qPCR analysis and normalized to GAPDH, β-actin and HPRT1. d Immunoblot analysis of HEK293T cells treated for 16 h using 2.5 µM lactacystin (LC). Cell lysates were prepared and the levels of the indicated proteins were examined by immunoblot analysis with β-actin used as a loading control. The data are expressed as the mean ± S.D. of three independent experiments. Statistical significance was determined using (b, c) the Student t test for unpaired observations or (a, d) one-way ANOVA (with Tukey multiple comparisons), where **P < 0.01 and ****P < 0.0001 are considered statistically significant

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