Fig. 7: Analyses of bone histomorphomertry and osteogenic activity of bone marrow mesenchymal cells. | Cell Death & Disease

Fig. 7: Analyses of bone histomorphomertry and osteogenic activity of bone marrow mesenchymal cells.

From: Chaperonin 60 sustains osteoblast autophagy and counteracts glucocorticoid aggravation of osteoporosis by chaperoning RPTOR

Fig. 7: Analyses of bone histomorphomertry and osteogenic activity of bone marrow mesenchymal cells.The alternative text for this image may have been generated using AI.

HSP60Tg mice showed minor responses to the glucocorticoid-induced loss of (a) BV/TV (scale bar, 120 μm), b Atg4, and Atg12 expressions, (c) RPTOR, and LC3-II levels, and LC3-II/LC3-I ratio. HSP60 overexpression (d) reduced levels of Bax and cleaved caspase3 but (e) increased LC3 immunostaining in osteoblasts (arrows) in glucocorticoid-treated bone tissue. Scale bar, 8 μm. HSP60 overexpression mitigated the glucocorticoid-induced loss of f Runx2, collagen 1α1, and osteocalcin expressions, and g mineralized nodule formation of bone marrow mesenchymal cells. Scale bar, 46 μm. Experiments were performed on six mice, and results are expressed as mean ± standard error. Asterisks (*) resemble a significant difference (P < 0.05) vs. wild-type group, and hashtag (#) indicates a distinguishable difference (P < 0.05) between the group of interest vs. glucocorticoid-treated wild-type group

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