Fig. 1: p53 and SIRT6 expression increases after palmitic acid (PA) treatment
From: p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis
a HCT116 cells were treated with PA (0.1 mM and 0.2 mM) or untreated (0 mM, Ctr) for 18 h and total p53 protein expression was detected by western blotting. β-actin was used as a loading control. b HCT116 cells were treated with 0.2 mM PA for 0, 12 and 18 h and the total p53 protein expression was detected by western blotting. c HCT116 cells were treated with various doses of PA and the protein expression of total SIRT1, SIRT6 and SIRT7 was detected by Western blotting. d HCT116 cells were treated with 0.2 mM PA for 0, 12 and 18 h, and total SIRT1, SIRT6 and SIRT7 expression was detected by western blotting. e, f HCT116 cells were harvested in NP-40 buffer to detect the soluble levels of p53 and SIRT6 by western blotting. g, h HCT116 cells were treated as outlined above, and p53, p21 and SIRT6 mRNA expression was analyzed by real-time PCR. mRNA levels of the control sample were set as 1, and relative mRNA levels of the experimental samples were normalized to this control. The bar (-) represents the means (n = 3)
