Fig. 6: IL-8 in GCMSC-CM promoted GC cells resistance to cytotoxic effect of CD8+ T cells. | Cell Death & Disease

Fig. 6: IL-8 in GCMSC-CM promoted GC cells resistance to cytotoxic effect of CD8+ T cells.

From: Gastric cancer mesenchymal stem cells derived IL-8 induces PD-L1 expression in gastric cancer cells via STAT3/mTOR-c-Myc signal axis

Fig. 6

a, b Cytotoxicity assay of GC cells. GC cells were treated with BMMSC-CM or GCMSC-CM for 24 h and then used for cytotoxicity assay. c, d Quantification of survival GC cell numbers. e, g Cytotoxicity assay of GC cells. IL-8 neutralizing antibody was added in GCMSC-CM and incubated at room temperature for 1 h and GCMSC-CM was then used to treat GC cells. After 24 h treatment, GC cells were used for cytotoxicity assay. f, h Quantification survival GC cell numbers. The concentration of PD-L1 neutralizing antibody was 2 μg/ml. Bar = 100 μm. Data in c, d, f and h represent the mean ± SD of three repeated experiments (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, n.s. not significant

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