Fig. 2: Differential expression of microRNAs and autophagy proteins in osteoarthritic cartilage. | Cell Death & Disease

Fig. 2: Differential expression of microRNAs and autophagy proteins in osteoarthritic cartilage.

From: MicroRNA-128a represses chondrocyte autophagy and exacerbates knee osteoarthritis by disrupting Atg12

Fig. 2

a Expression of seven microRNAs was significantly upregulated and b that of seven other microRNAs significantly downregulated in injured cartilage at 8 weeks after ACLT. c Expression of miR-128 was increased throughout the study. d In situ hybridization further revealed strong expression of miR-128a in articular chondrocytes and synovial fibroblasts of the ACLT group. Data are expressed as the mean ± SEM calculated from eight rats. Asterisks (*) indicate P < 0.05 between groups. e Inverse relationship between increased expression of miR-128a and downregulation of Atg12 mRNA in cartilage from patients with end-stage OA. Figures shows in situ hybridization of miR-128a and immunostaining of LC3 and Atg12, respectively (left panels), next to quantification of relative expression of miR-128a and Atg12 mRNA probed by RT-PCR and immuno-intensities in vertical scatter plots (right panels). Data are calculated from 28 patients with end-stage knee OA and 17 patients with femoral neck fracture (non-OA). Asterisks (*) indicate P < 0.05 between groups

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