Fig. 4: PHGDH inhibition regulates Oct4 at post-translational levels in ECSLCs. | Cell Death & Disease

Fig. 4: PHGDH inhibition regulates Oct4 at post-translational levels in ECSLCs.

From: Phosphoglycerate dehydrogenase inhibition induces p-mTOR-independent autophagy and promotes multilineage differentiation in embryonal carcinoma stem-like cells

Fig. 4

a NT2/D1 cells, with scrambled control or PHGDH shRNA, were subjected to qRT-PCR analysis for POU5F1(Oct4) mRNA levels. b NT2/D1 cells, with scrambled control or PHGDH shRNA, were subjected to (i) WB analysis for total levels of ubiquitin, and (ii) immunoprecipitation (IP) using anti-Oct4 antibody followed by WB analysis for specific ubiquitination of Oct4. c NT2/D1 cells, with scrambled control or PHGDH shRNA, were treated with MG132, and subjected to WB analysis for (i) Oct4, and (ii) total levels of ubiquitin. d, e NT2/D1 cells, with scrambled control or Oct4 shRNA, were subjected to d WB and e qRT-PCR analysis for PHGDH/PHGDH, respectively. * p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001

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