Fig. 7: PHGDH inhibition promotes Beclin-1-dependent canonical autophagy mediated by p-AMPK in a p-mTOR-independent manner. | Cell Death & Disease

Fig. 7: PHGDH inhibition promotes Beclin-1-dependent canonical autophagy mediated by p-AMPK in a p-mTOR-independent manner.

From: Phosphoglycerate dehydrogenase inhibition induces p-mTOR-independent autophagy and promotes multilineage differentiation in embryonal carcinoma stem-like cells

Fig. 7

a NT2/D1 cells, with scrambled control or PHGDH shRNA, were subjected to western blot (WB) analysis for (i) p-mTOR, mTOR, p-Akt and Akt, (ii) p-p70S6K, p70S6K, p-4EBP1, 4EBP1. b NT2/D1 cells, with scrambled control or PHGDH shRNA, were treated with rapamycin and subjected to WB analysis for (i) SQSTM1, LC3A-II, LC3B-II, and (ii) caspase-3. c NT2/D1 cells, with scrambled control or PHGDH shRNA, were subjected to WB analysis for p-AMPK, p-TSC2, TSC2, p-Beclin-1. d Schematic diagram depicting the activation of autophagy through AMPK-Beclin-1 activation due to energy depletion. e NT2/D1 cells, with scrambled control or siBeclin-1 siRNA, were treated with PHGDH inhibitor CBR-5884 and chloroquine (CQ), alone or in combination, and the levels of LC3A-II and LC3B-II were analyzed by WB

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