Table 1 Primer sequences of target genes

From: Vascularization converts the lineage fate of bone mesenchymal stem cells to endothelial cells in tissue-engineered bone grafts by modulating FGF2-RhoA/ROCK signaling

Gene

Forward

Reverse

Osterix

5′-TGACTGCCTGCCTAGTGTCTACA-3′;

5′-TGGATGCCCGCCTTGT-3′

RUNX2

5′-CCGATGGGACCGTGGTT-3′

5′-CAGCAGAGGCATTTCGTAGCT-3′

CD31

5′-GAACAAACTTGCAAGGAGCAGGAA-3′

5′-CACGGAGCAAGAAAGACTCTGA-3′

VEGF

5′-GCTCTCTTGGGTGCACTGGA-3′

5′-CACCGCCTTGGCTTGTCACA-3′

RhoA

5′-ACTCGGAGTCCTCGCCTTGA-3′

5′-TCTGGGAACTGGTCCTTGCTG-3′

ROCK-1

5′-CTGGACATTTGAAGTTAGCCG-3′

5′-CCAACTGCTGTATCACATCGTACC-3′

ROCK-2

5′-CCCGATCATCCCCTAGAACC-3′

5′-TTGGAGCAAGCTGTCGACTG-3′

FGF2

5′-TTCCCACCCGGCCACTTCAAG-3′

5′-GTTCGCACACACTCCCTTGA-3′

GAPDH

5′-CAGCAAGGATACTGAGAGCAAGAG-3′

5′- GGATGGAATTGTGAGGGAGATG-3′

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