Fig. 2: Loperamide, pimozide, and STF-62247 induce autophagy-dependent cell death of MZ-54 in a time-dependent manner.
From: Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells
a–d MZ-54 cells were treated with 17.5 µM loperamide, 15 µM pimozide, 40 µM STF-62247, and 20 µM IM/100 µM TIC for 24, 48, and 72 h. Cell death was assessed by measuring the PI uptake as fraction of total nuclei determined by Hoechst counterstaining using high-content fluorescence microscopy. Mean and SEM of 3−5 independent experiments performed in triplicate are shown. Significances are calculated versus WT cells. *p < 0.05, **p < 0.01, ***p < 0.001. LOP loperamide, PIMO pimozide, STF STF-62247, IM imipramine hydrochloride, TIC ticlopidine
