Fig. 3: Ngb overexpression promotes neuronal differentiation of cultured NPC.
From: Neuroglobin promotes neurogenesis through Wnt signaling pathway
NPC was cultured in differentiation medium (DMEM/F12 + 2%FBS) and Lentivirus containing Ngb or GFP gene was transduced on day 2 of culture. Differentiated cells were examined by immunocytochemistry using anti-Tuj1 (immature neuron maker) and anti-GFAP antibodies. NPC differentiation was examined by immunostaining for Tuj1 (immature neuron) and GFAP (astrocytes). a Representative images of Tuj1 and GFAP immunostaining; b Quantification of percentages Tuj1 positive cells, c Quantification of average neurite length (n = 4,* p < 0.05 vs Lv-GFP)
