Fig. 4: Wnt signaling is involved in the promotional effect of Ngb overexpression on neurogenesis.
From: Neuroglobin promotes neurogenesis through Wnt signaling pathway
To investigate the mechanisms of the neurogenesis promotional effect by Ngb overexpression, we examined the protein levels of Dvl1 and β-catenin, the two key components of Wnt signaling, after Lv-Ngb transduction in cultured neurosphere. a Representative Western blot images for Dvl1 and β-catenin using cell lysate of cultured NPCs. β-actin was used as loading control. b Quantification of Dvl1 protein level; c Immunostaining for β-catenin in cultured NPC; d Representative Western blot images and e quantification of β-catenin in isolated nuclear proteins. Histone-H3 was used as nucleus loading control (n = 4,* p < 0.05 vs Lv-GFP). We further used a Wnt inhibitor, IWR-endo, to test the involvement of Wnt signaling in the neurogenesis promotional effect by Ngb overexpression; f Relative neurosphere number after Lv-Ngb transduction and IWR-endo treatment; g Relative neurosphere size after Lv-Ngb transduction and IWR-endo treatment; h The percentage of Tuj positive cell numbers in NPC differentiation after Lv-Ngb transduction and IWR-endo treatment (n = 4, *p < 0.05 vs Lv-GFP, #p < 0.05 vs Lv-Ngb)
