Fig. 5: Ngb overexpression improved neurogenesis in mice after MCAO.
From: Neuroglobin promotes neurogenesis through Wnt signaling pathway
To test the effect of Ngb overexpression on neurogenesis after stroke, we performed transient MCAO in mouse and administered Lv-Ngb or Lv-GFP through ICV injection at 2 days after stroke. a Ngb protein level was measured by Western blot to confirm Ngb overexpression by Lv-Ngb transduction at 5 days post stroke. NPC proliferation was assessed at 7 days post stroke using PSA-NCAM immunostaining. b PSA-NCAM immunostaining in SVZ area; c Quantification of PSA-NCAM positive cells in SVZ regions; d PSA-NCAM immunostaining in peri-infarct cortex region; e Quantification of PSA-NCAM positive cells in SVZ regions. Furthermore, differentiated immature neurons were assessed at 10 days post stroke using Tuj1 immunostaining. f Tuj1 immunostaining in hippocampus region; g Quantification of Tuj1 positive cells in hippocampus region; h Tuj1 immunostaining in peri-infarct cortex region; i Quantification of Tuj1 positive cells in peri-infarct cortex area. (n = 6,* p < 0.05 vs sham, #p < 0.05 vs MCAO Lv-GFP)
