Fig. 1: CCN6 increased chondrosarcoma cell migration and invasion, and enhanced cellular MMP-9 expression.
From: CCN6-mediated MMP-9 activation enhances metastatic potential of human chondrosarcoma
a, b Cells were incubated with CCN6 (10–100 ng/mL), and a Transwell assay determined in vitro migratory and invasion activity after 24 h. c, d Cells were incubated with CCN6 (10–100 ng/mL) for 24 h, then MMP-9 mRNA and protein expression was examined by RT-qPCR, zymography, and Western blot. e, f Cells were transfected with MMP-9 siRNA for 16 h then stimulated with CCN6 (100 ng/mL); migration and invasion potential was measured with the Transwell assay. g MMP-9 protein levels were measured by Western blot. Quantitative results are expressed as the mean ± SEM. *p < 0.05 as compared with the control group; #p < 0.05 as compared with the CCN6-treated group
