Fig. 4: CCN6 induces cell migration, invasion, and MMP-9 upregulation via NF-κB.
From: CCN6-mediated MMP-9 activation enhances metastatic potential of human chondrosarcoma
a Cells were pretreated for 30 min with PDTC (10 μM) and TPCK (3 μM) or transfected with p65 siRNA for 16 h, followed by stimulation with CCN6. a, b Cell migration and invasion were examined using the Transwell assay. (c) MMP-9 mRNA expression was examined by RT-qPCR. d JJ012 cells were incubated with CCN6 for the indicated time intervals, and p-IKKα/β, p-IκB, and p-p65 levels were examined by Western blotting. e JJ012 cells were pretreated with Ly294002, wortmannin, an Akt inhibitor, or rapamycin for 30 min, then stimulated with CCN6 for 60 min, and p65 phosphorylation was examined. Quantitative results are expressed as the mean ± SEM. *p < 0.05 as compared with the control group; #p < 0.05 as compared with the CCN6-treated group
