Fig. 4: Involvement of Caspase-11 and IL-1β in contrast-induced acute kidney injury.

a Representative images (six visual fields for each tissue sample analyzed) of immunolabelling for caspase-11 in the indicated experimental groups. Scale bars, 100 μm. b Quantitative analysis for caspase-11-positive area in the kidneys by ImageJ. c Immunoblot analysis of caspase-11 and cleaved caspase-11 (p20) showed that iohexol (10 μl/g) injection by tail vein markedly increased the expression of caspase-11 and p20, as compared with that in the Ctrl, model, and model + NS groups. d Quantification of the caspase-11 and p20 expression shows significant upregulation in WT mice injected with iohexol (10 μl/g), compared with that in the Ctrl, model mice, and mice injected with NS. e Representative images (six visual fields for each tissue sample analyzed) of immunolabeling for mature IL-1β in the indicated experimental groups. Scale bars, 100 μm. f Quantitative analysis for mature IL-1β-positive area in the kidneys by ImageJ. g Immunoblot analysis of pro-IL-1β and mature IL-1β showed that iohexol (10 μl/g) injection by tail vein markedly increased the expression of mature IL-1β, as compared with that in the Ctrl, model, and model + NS groups. h Quantification of the pro-IL-1β and mature IL-1β expression shows significant upregulation in WT mice injected with iohexol (10 μl/g), compared with that in the Ctrl, model, and model + NS groups. Renal tissue samples were collected at 24 h after injection of iohexol (10 μl/g) or NS via tail vein. Ctrl control, NS normal saline, WT wild type. ***P < 0.001 vs. control or as indicated. Data are means ± SEM from five to six mice per group. Statistics obtained from ANOVA