Fig. 2: STAT1 is required for EGF-induced apoptosis in metastatic breast cancer cells. | Cell Death & Disease

Fig. 2: STAT1 is required for EGF-induced apoptosis in metastatic breast cancer cells.

From: Biased signaling downstream of epidermal growth factor receptor regulates proliferative versus apoptotic response to ligand

Fig. 2

a Lymph node metastases (NME-Lym1 and NME-Lym2) were stimulated with EGF (100 ng/ml) for 36 h and imaged via phase contrast microscopy. b Following EGF stimulation as described in panel a, cells were assayed for caspase 3/7 activity. c NME-Lym1 cells were constructed to stably express a scrambled control (sc) shRNA or various shRNAs (23–27) targeting STAT1. These cells were analyzed for STAT1 expression by immunoblot. Expression of β-tubulin (β-tub) served as a loading control. d Control (shscram) and STAT1 depleted (shSTAT1#23 and shSTAT1#24) NME-Lym1 cells were treated with EGF as described above and caspase 3/7 activity was assessed. Separate groups of cells were treated with the MEK1/2 inhibitor trametinib (100 nM) in the presence or absence of exogenous EGF (100 ng/ml) and these cells were similarly assayed for caspase 3/7 activity. Data in panels b and c are the mean ± SE of three independent experiments completed in triplicate resulting in the indicated P values

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