Fig. 1: Expression of AFP and its interaction with PTEN in PLC/PRF/5 and HLE cells. | Cell Death & Disease

Fig. 1: Expression of AFP and its interaction with PTEN in PLC/PRF/5 and HLE cells.

From: Alpha-fetoprotein inhibits autophagy to promote malignant behaviour in hepatocellular carcinoma cells by activating PI3K/AKT/mTOR signalling

Fig. 1: Expression of AFP and its interaction with PTEN in PLC/PRF/5 and HLE cells.

a Western blotting of AFP, PTEN and LC3 expression in PLC/PRF/5 and HLE cells. b Co-localization of AFP and PTEN in PLC/PRF/5 cells. Localization of AFP and PTEN was observed on a laser scanning confocal microscope. Nuclei were stained with DAPI (blue). AFP and PTEN were labelled with TRITC (red) and FITC (green), respectively. c The interaction of AFP and PTEN was analysed using co-immunoprecipitation. Lysates from PLC/PRF/5 cells were immunoprecipitated (IP) with antibodies against AFP or PTEN and separated by SDS/PAGE. d FRET measurement for Alexa 568-AFP (Donor) and Alexa 488-PTEN (Acceptor) in PLC/PRF/5 cells. (a) Pre-bleaching; (b) Bleaching; (c) Post-bleaching; (d) Bleaching results; Right panel: Bleaching efficiency. All images are representative of at least three independent experiments

Back to article page