Fig. 4: PAC-1 induces the DNA damage response, arrests cell cycle progression and blocks DNA synthesis. | Cell Death & Disease

Fig. 4: PAC-1 induces the DNA damage response, arrests cell cycle progression and blocks DNA synthesis.

From: Procaspase-3-activating compound 1 stabilizes hypoxia-inducible factor 1α and induces DNA damage by sequestering ferrous iron

Fig. 4

A, a Fluorescence images of RAD51 foci and concentration-dependent increases in the total areas of RAD51 foci after RAD51-EGFP_SW480 cells were treated with PAC-1 for 24 h. B, b Fluorescence images of accumulated pH2AX and the concentration-dependent increase in pH2AX intensity in HepG2 cells treated with PAC-1 for 24 h. C, c Cell cycle distribution in HepG2 cells after a 24-h PAC-1 treatment, as measured by flow cytometry. D, E BrdU incorporation status after PAC-1 exposure in HepG2 and U2OS cells. Cells were exposed to PAC-1 for 3 h before the addition of BrdU for another 3 h. Cells undergoing DNA synthesis within the last 3 h were stained with a BrdU-specific fluorescent dye (red). Images were generated by merging the DAPI and BrdU channels. d, e The EC50 was calculated using the data for BrdU intensity. Data represent means ± SEs of three independent experiments. **P < 0.01 and *P < 0. 05 compared with the corresponding control

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