Fig. 2: The presence of BMPR1A at the cell surface of AML samples is induced by BMP4 and BMPR1A and is associated with the presence of very immature cells. | Cell Death & Disease

Fig. 2: The presence of BMPR1A at the cell surface of AML samples is induced by BMP4 and BMPR1A and is associated with the presence of very immature cells.

From: A new signaling cascade linking BMP4, BMPR1A, ΔNp73 and NANOG impacts on stem-like human cell properties and patient outcome

Fig. 2

a Percentage of mononuclear cells (MNCs) of healthy donors (n = 8) and AML (n = 11) samples expressing BMPR1A at their membrane determined by flow cytometry. b Correlation between the expressions of BMPR1A and Id1 in AML samples at diagnosis (n = 50). c Relative expression of BMPR1A in MNCs from healthy donors (n = 6) and AML patients (n = 9), exposed or not, to BMP4 (10 ng/mL) for 24 h. d Effect of BMP4 (10 ng/mL) exposure on the proportion of BMPR1A-positive cells at the surface in healthy and AML MNC samples (n = 6 patients for each cohort). e Effect of BMP4 (10 ng/mL) exposure for 24 h on the proportion of very immature leukemic cells in AML mononuclear cells (MNCs), evaluated by long-term culture-initiating cells (LTC-IC) assay (n = 3). f Flow cytometric analysis of the expression of BMPR1A at the cell surface according to CD34 and CD38 status of MNCs from an AML patient. g Number of colony-forming cells (CFC) and LTC-IC among peripheral blood MNCs from AML patients according to their low (n = 8) or high (n = 6) BMPR1A mRNA level. h AML samples with low (n = 6) or high (n = 7) BMPR1A mRNA levels were analyzed for ALDH activity by flow cytometry. NT non-treated, NBM normal bone marrow. Statistical analysis: P values were determined using Spearman’s nonparametric test (b); Wilcoxon matched-pairs signed rank test (c, d); *P < 0.05; **P < 0.01

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