Fig. 4: TRPC6 inhibition mitigates H₂O₂-induced apoptosis in primary PTC.

a PTC isolated from WT mice were treated with H₂O₂ (0.5 mM) for different times. The viability and LDH release of PTC was measured. All data are expressed as mean ± SEM, n = 6; *P < 0.05. b Representative western blot images and the relative quantification of cleaved caspase-3 (CC3). Data are expressed as mean ± SEM, n = 4; *P < 0.05. c PTC isolated from WT mice were treated with H₂O₂ (0.5 mM) in the absence and presence of SAR7334 (100 nM) for 12 h. The viability and LDH release of PTC was measured. All data are expressed as mean ± SEM, n = 3; *P < 0.05 vs. control, ^#P < 0.05 vs. the H₂O₂ group. d Representative western blot images of CC3 after treatment with H₂O₂ (0.5 mM) in the absence and presence of SAR7334 (100 nM) for 12 h. Bar graph is showing the relative quantification of CC3. Data are expressed as mean ± SEM, n = 3; *P < 0.05 vs. control, ^#P < 0.05 vs. the H₂O₂ group. e PTC were treated with H₂O₂ (0.5 mM) in the absence and presence of SAR7334 (100 nM) for 12 h. Mitochondrial membrane potential was measured using JC-1 dye. Bar diagram is showing the number of mPT (mitochondrial permeability transition)-positive cells upon H₂O₂ treatment. Data are expressed as mean ± SEM, n = 3; Scale Bar = 50 μm, *P < 0.05 vs. control, ^#P < 0.05 vs. the H₂O₂ group