Fig. 6: Blockage of autophagy prevents the protective effect of TRPC6 knockout. | Cell Death & Disease

Fig. 6: Blockage of autophagy prevents the protective effect of TRPC6 knockout.

From: Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation

Fig. 6

PTC isolated from WT or TRPC6-/- mice were divided into eight different groups and treated with H2O2 (0.5 mM) in the absence and presence of CQ (25 μM) for 12 h. a Representative TUNEL staining of PTC in each group, Scale Bar = 50 μm. Bar graph is showing the quantification of TUNEL-positive cells. Data are expressed as mean ± SEM, n = 6; *P < 0.05. b Representative flow cytometric assessment of apoptosis via double-staining with Annexin V-FITC and PI. Bar diagram is showing the apoptosis rates of different groups. Data are expressed as mean ± SEM, n = 3; *P < 0.05

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