Fig. 6: NHE1 blockade in combination with TMZ treatment increases antitumor pro-inflammatory TAMs.

a Starting 2 d.p.i. of SB28 cells implantation, mice received either vehicle PBS-DMSO, HOE642, TMZ, or T+H combination treatments for 5 consecutive days. Mice were sacrificed at 15 d.p.i. and flow cytometric analysis of tumor tissues was performed. b Representative flow cytometric profile showing gating strategy of microglia (CD11b⁺/CD45^low-medium) and infiltrating myeloid cells (CD11b⁺/CD45^hi). c Total number of CD11b⁺/CD45^low-medium and CD11b⁺/CD45^hi cells. d Inflammatory profile of CD11b⁺/CD45^low-medium and CD11b⁺/CD45^hi cells. All data are mean ± SEM, n = 5–6, ^#p ≤ 0.05 vs. Veh-control, *p ≤ 0.05