Fig. 1: ChrEtOH perturbs mitochondrial morphology and dynamics significantly only in VL-17A cells. | Cell Death & Disease

Fig. 1: ChrEtOH perturbs mitochondrial morphology and dynamics significantly only in VL-17A cells.

From: Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism

Fig. 1

Cells were transfected with mitochondrial matrix-targeted DsRed (mtDsRed) and mtPA-GFP. Mitochondrial morphology and GFP spreading after photoactivation in designated areas are shown for a typical control HepG2 a and VL-17A cells b or the cells chronically treated with EtOH c, d. In the lower panels, only the green channel is shown. e, f Spreading rate of GFP after photoactivation has been quantified and presented as the normalized ratio of mtPA-GFP to mitoDsRed in the photo-bleached regions. e, f fusion rates were presented as the mean number of fusion events that calculated manually in the region of interests (ROI) for 450 s. e n for control and EtOH is 27 and 29; f n for both HepG2 and EtOH is 37) (n = 4)

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