Fig. 3: ChrEtOH exposure induces ROS increase in the mitochondria of VL-17A cells but not in HepG2 cells. | Cell Death & Disease

Fig. 3: ChrEtOH exposure induces ROS increase in the mitochondria of VL-17A cells but not in HepG2 cells.

From: Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism

Fig. 3

a A representative time series recorded for oxidized glutathione (GSSG) to reduced glutathione (GSH) ratio using cytosolic Grx1-roGFP2. The trace was normalized to DTT response as minimum and H2O2 as maximum (zero and one). b Mean±SE of peak fluorescence ratio of cytosolic Grx1-roGFP2 values obtained from normalized individual traces is shown. N was 62 and 59 for untreated and chronically treated HepG2 cells and 13 and 20 for untreated and chronically treated VL-17A. c The same as B except that the probe is localized in mitochondria. N was 74 and 58 for untreated and chronically treated HepG2 cells and was 20 and 24 for untreated and chronically treated VL-17A (n = 4, *: p < 0.05)

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