Fig. 5: ΔΨm, Ca2+ handling, and the abundance of the mitochondrial fusion proteins are unchanged in chronically EtOH-treated VL-17A and HepG2 cells. | Cell Death & Disease

Fig. 5: ΔΨm, Ca2+ handling, and the abundance of the mitochondrial fusion proteins are unchanged in chronically EtOH-treated VL-17A and HepG2 cells.

From: Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism

Fig. 5

a ΔΨm before and after the addition of Ca2+ were presented as the fluorescent intensity of TMRM normalized to the TMRM fluorescent intensity obtained after complete depolarization by FCCP (5 μM) in permeabilized cell suspensions (n = 3). b Mitochondrial Ca2+ uptake was measured using Fura2FF and the data presented as the nmol Ca2+ (n = 3). c Western blot for Mfn1/2 and Opa1 in the cell lysates of VL-17A and HepG2 cells, which were chronically treated with EtOH. Actin was used as loading control (n = 3)

Back to article page