Fig. 3: Hypoxia-induced PAECs proliferation and migration were inhibited by GW4869 in vitro.
From: Exosomal 15-LO2 mediates hypoxia-induced pulmonary artery hypertension in vivo and in vitro
a Immunofluorescence staining of Ki67 indicated that GW4869 repressed the hypoxia-induced PAECs growth. Scale bar = 50 μm. b The protein expression of PCNA was determined by western blot analysis. c Proliferation of PAECs was evaluated by the MTT and BrdU incorporation assays. d The tube formation and migration of PAECs were examined after GW4869 treatment. Scale bar = 100 μm. e, f The number of cells in each phase of the cell cycle was examined by flow cytometry, the protein expression levels of CyclinA, CyclinE, CyclinD, CDK2, and CDK4 were tested under normoxia or hypoxia. Hyp, Hypoxia; Nor, normoxia; H+GW, hypoxia plus GW4869; *p < 0.05, **p < 0.01, and ***p < 0.001. All values are presented as mean ± SD
