Fig. 5: 15-LO2-containing exosomes modulated the phosphorylation of STAT3.
From: Exosomal 15-LO2 mediates hypoxia-induced pulmonary artery hypertension in vivo and in vitro
a After treatment with GW4869, the phosphorylation of STAT3 and c-Myc were examined by western blot analysis. b Immunofluorescence analysis of p-STAT3 was done after GW4869 administration. c PAECs were treated with NDGA or 15-HETE, and the protein levels of p-STAT3 and c-Myc were assessed by western blot analysis. d The colocalization of 15-LO2 and p-STAT3 was determined with immunofluorescence assay. e PAECs were exposed to normoxia or hypoxia for 24 h and the whole cell lysates were extracted for co-immunoprecipitation with anti-15-LO2 or anti-p-STAT3, followed by probing with anti-p-STAT3 or anti-15-LO2, respectively. Nor, normoxia; Hyp, hypoxia; H+GW, hypoxia plus GW4869; H+NDGA, hypoxia plus NDGA; H+NDGA+15, hypoxia plus NDGA and 15-HETE; H+15, hypoxia plus 15-HETE. Scale bar = 50 μm.*p < 0.05, **p < 0.01, and ***p < 0.001. All values are presented as mean ± SD
