Fig. 5: MiR-146b directly targeted FBXL10. | Cell Death & Disease

Fig. 5: MiR-146b directly targeted FBXL10.

From: miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer

Fig. 5

a Schematic representation of the miR-146b and its targeting sites in the 3′-UTR of FBXL10, indicating the binding sites and the corresponding mutations. b miR-146b directly interacted with the FBXL10-3′-UTR. The 293T cells were co-transfected with the luciferase reporter vector, and pcDNA3.1-pri-miR-146b or control plasmids into 293T cells. The luciferase activity was measured after 24 h according to the protocol described in Materials and methods section. c Levels of mature miR-146b were detected using qPCR after transfection with miR-146b mimics or miR-146b inhibitors for 24 h. d, e mRNA (d) and protein (e) levels of FBXL10 in HO8910 and SKOV3 cells transfected with miR-146b mimics or miR-146b inhibitors. f Relative levels of H3K4me3 and H3K36me2 transfected with miR-146b mimics or miR-146b inhibitors. g, h The expression level of FBXL10 in ovarian cancer samples using qPCR (g) and immunohistochemical staining (h) (control samples, n = 21; cancer samples, n = 22). i Regression and correlation analysis between miR-146b and FBXL10 expression in ovarian cancers (n = 37). Scale bars represent 100 μm. *p < 0.05, **p < 0.01, and ***p < 0.001; ns not significant

Back to article page