Fig. 3: Radiation-induced DNA damage and its repair in unstimulated compared with stimulated PBLCs. | Cell Death & Disease

Fig. 3: Radiation-induced DNA damage and its repair in unstimulated compared with stimulated PBLCs.

From: Sensitivity of CD3/CD28-stimulated versus non-stimulated lymphocytes to ionizing radiation and genotoxic anticancer drugs: key role of ATM in the differential radiation response

Fig. 3

a DNA double-strand break repair kinetics determined by neutral comet assay at different time points directly (0 min) and up to 240 min after 5 Gy (n = 3, 50 cells per sample, mean value, SD). b γH2AX-kinetic in non-irradiated (0 h) and 1 Gy-irradiated PBLCs 1–24 h post treatment. c DNA single-strand breaks 1 min after different doses of IR were analyzed by the alkaline comet assay (n = 3, 50 cells per sample, mean value, SD). d DNA single-strand break repair kinetics determined by alkaline comet assay at different time points directly (0 min) and up to 240 min after 5 Gy (n = 3, 50 cells per sample, mean value, SD). Representative pictures of neutral and alkaline comets are available in Figure S6a, b

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