Fig. 4: Induction of p53 and cleavage of caspases in unstimulated and stimulated PBLCs after 1 Gy irradiation (western blottings). | Cell Death & Disease

Fig. 4: Induction of p53 and cleavage of caspases in unstimulated and stimulated PBLCs after 1 Gy irradiation (western blottings).

From: Sensitivity of CD3/CD28-stimulated versus non-stimulated lymphocytes to ionizing radiation and genotoxic anticancer drugs: key role of ATM in the differential radiation response

Fig. 4

a, b Expression and phosphorylation of p53 at Serin 46 and Serin 15. There were no differences in radiation-induced p53 between unstimulated and stimulated PBLC detectable. However, p53 expression was slightly induced upon CD3/CD28 stimulation (control, stim PBLC) (n = 3). c Quantification of Fas receptor in non-irradiated and irradiated PBLCs (n = 3, t-test *p < 0.05, **p < 0.01). Stimulated PBLC showed a significant higher level of Fas expression compared with unstimulated cells, independent from exposure to IR. d Expression and cleavage of initiator caspases-8 and -9, and executive caspases-3 and -7 in stimulated PBLCs, control, and following IR (controls) (n = 3). e Caspase-2 was cleaved in both unstimulated and stimulated PBLCs (n = 2). f In unstimulated and stimulated PBLCs, no cleavage of caspase-1 was visible. Gasdermin D, a substrat of caspase-1 processed in the cell death pathway named pyroptosis, was not cleaved after exposure to IR (n = 3). In all western blottings, ERK2 was used as loading control

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