Fig. 2: MITF exhibits tumor-suppressive activity in PC3 PCa cell line. | Cell Death & Disease

Fig. 2: MITF exhibits tumor-suppressive activity in PC3 PCa cell line.

From: Integrative analysis of transcriptomics and clinical data uncovers the tumor-suppressive activity of MITF in prostate cancer

Fig. 2

a, b Analysis and quantification of MITF expression by qRTPCR (a, n = 8)) and western blot (b, representative experiment out of three independent ones) in PC3 TRIPZ-MITFA cells after treatment with 0.5 μg mL−1 doxycycline (Dox). c Relative cell number quantification by crystal violet in doxycycline-treated and nontreated PC3 TRIPZ-MITFA cells. Data are normalized to day 0. Asterisks indicate statistics of five independent experiments. One representative experiment out of five is shown. Error bars represent standard deviation. d, e Effect of MITF induction on anchorage-independent growth (d, soft agar; n = 4 independent experiments) and BrdU incorporation (e, n = 3 independent experiments). f Impact of MITF induction in tumor growth rate of PC3 TRIPZ-MITFA cells (n = 7 animals per group; 14 injections/tumors). No dox: MITFA noninduced conditions; Dox: MITFA-induced conditions. Error bars represent s.e.m. (a, d, and e) or minimum and maximum values (f). Statistic test: One-sample t test (a, d, and e) and Student’s t test (c, f *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page