Fig. 5: Role of the MAPK pathway in the activity of Triapine and Me2NNMe2. | Cell Death & Disease

Fig. 5: Role of the MAPK pathway in the activity of Triapine and Me2NNMe2.

From: The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition

Fig. 5: Role of the MAPK pathway in the activity of Triapine and Me2NNMe2.

a GSEA from whole-genome gene expression data revealed significant enrichment of genes in the “MAPK signaling pathway” gene set in SW480 cells treated with 0.1 or 1 µM Me2NNMe2 compared to untreated cells. Normalized enrichment score (NES) and false discovery rate (FDR) are given. b Illustration of genes up- (red) or down-regulated (blue) in the KEGG-derived “MAPK signaling pathway” of Me2NNMe2 (1 µM)-treated compared to untreated SW480 cells using whole-genome gene expression data. c Western blot analysis of MEK1/2 and ERK1/2 as well as their phosphorylated protein levels in SW480 and HCT-116 cells treated with indicated concentrations of Triapine and Me2NNMe2 for 24 h. β-actin was used as a loading control

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