Table 3 Primer sequences of genes in the study

PLAGL2

Forward: 5′-AAAGCAGGAGGAGGAAGTGG-3′

Reverse: 5′-TTTCTGGGCTGAGTGGGTGG-3′

ARID4B

Forward: 5′-GGCATAGGTTATTTCCGTGGTA-3′

Reverse: 5′-CTTTGGCACATTTTTATCAGCA-3′

GAPDH

Forward: 5′-GAAGGTGAAGGTCGGAGTC-3′

Reverse: 5′-GAAGATGGTGATGGGATTTC-3′

MSP (methylation)

Forward: 5′-CGTCGATGTCGTTTAGGAGTAC-3′

Reverse: 5′-GCGAACGCCTCAATACCT-3′

MSP (unmethylation)

Forward: 5′-TAGTGTTGATGTTGTTTAGGAGTAT-3′

Reverse: 5′-ACCACAAACACCTCAATACCT-3′

BSP

Forward: 5′-AGGGTTTTATTATGTTGGTTAGG-3′

Reverse: 5′-CCTCAATACCTTAAAATATTTCATAAA-3′