Fig. 3: PARP1 mediates nuclear localization of FoxO3a.
From: PARP1 promote autophagy in cardiomyocytes via modulating FoxO3a transcription
a–c Primary neonatal rat cardiomyocytes were starved for indicated different times. Western-blot assay was used to detect the expression of FoxO3a in total extracts (a), nuclear extracts (b) or cytoplasm extracts (c), respectively. Primary rat cardiomyocytes were pre-treated with 3AB (10 mM,) or PJ34 (10 µM), or transfected with PARP1 shRNA or an unrelated shRNA for 24 h followed by starvation (24 h). d Confocal immunofluorescence assay was used to detect the expression of FoxO3a (red fluorescence), Hoechst (blue fluorescence) was used to stain the cell nuclei (scale bar = 40 µm). e and f Western-blot assay was used to detect the expression of total FoxO3a and pFoxO3a
