Fig. 6: CpG Methylation of PTGIS promoter region plays an important role in transcriptional regulation of HIF-1α. | Cell Death & Disease

Fig. 6: CpG Methylation of PTGIS promoter region plays an important role in transcriptional regulation of HIF-1α.

From: CIRBP is a novel oncogene in human bladder cancer inducing expression of HIF-1α

Fig. 6

a MethHc database showed that PTGIS has a high DNA methylation levels in BCa compared with normal samples. b Bisulfite sequence analyses of 36 CpG sits of PTGIS promotor region in two BCa cell lines (UM-UC-3 and 5637) and immortalized normal uroepithelial cell line (SV-HUC-1). c qRT-PCR analysis detected the mRNA level of PTGIS in distinct malignancy BCa cells (T24, UM-UC-3, 5637, BIU-87) and immortalized normal uroepithelial cell line (SV-HUC-1). d qRT-PCR analysis detected the mRNA level of PTGIS in UM-UC-3 cells after the treatment by the demethylating agent 5-Aza (Cayman Chemical #11166-5) at 0, 0.5 and 1 μM for 24 h. e qRT-PCR analysis detected the effects of HIF-1A overexpression on PTGIS in SV-HUC-1 cells. f qRT-PCR analysis detected the effects of HIF-1A overexpression on PTGIS in demethylating UM-UC-3 cells (pre-treatment with 1 µM 5Aza for 24 h)

Back to article page