Fig. 2: Results from the synergy screen with docetaxel. | Cell Death & Disease

Fig. 2: Results from the synergy screen with docetaxel.

From: A functional genomics screen reveals a strong synergistic effect between docetaxel and the mitotic gene DLGAP5 that is mediated by the androgen receptor

Fig. 2

a Heatmap illustrating the results from the docetaxel (DCT) synergy screen. Columns are sorted as before. The colours show %GI under DCT relative to DMSO (window). A full red colour indicates a positive window of 50 percentage points, a green colour indicates a negative window. b Individual data for CDC20, TPX2 and DLGAP5. In blue, growth inhibition in DMSO, relative to NTC (four siRNAs, three cell lines; shown is mean of experiments + SEM, n = 3). In green, growth inhibition in DCT relative to NTC in DCT. c, d, e Knockdown validation by western blot, performed in PC3 cells for the 4 individual siRNAs targeting CDC20 (c, e top panels) and performed in LNCaP cells for the siRNAs targeting DLGAP5 (d, e bottom panels). f, g Validation of growth inhibition by Incucyte growth assay. Growth was measured by area of confluence, values shown are treatment condition relative to DMSO condition, mean of experiments + SEM (n = 2). CDC20 validation was performed in PC3 cells (f), DLGAP5 validation was performed in LNCaP cells (g)

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