Fig. 3: Nimbolide triggers autophagy in oral cancer cells.

a Cells treated with nimbolide for 12 and 24 h were stained with acridine orange. Representative images of acridine orange-stained acidic vesicular organelles captured by fluorescence microscope (×20) were shown. b Western blot showing expressions of LC-3 I/II, ATG5, p62 and Beclin-1 in cells treated with nimbolide for 12 and 24 h. β-actin was used as loading control