Fig. 4: Crosstalk between autophagy and apoptosis upon nimbolide treatment. | Cell Death & Disease

Fig. 4: Crosstalk between autophagy and apoptosis upon nimbolide treatment.

From: Nimbolide, a neem limonoid inhibits cytoprotective autophagy to activate apoptosis via modulation of the PI3K/Akt/GSK-3β signalling pathway in oral cancer

Fig. 4

a SCC131 and SCC4 cells were treated with nimbolide from 0 to 96 h. Expression of key molecules involved in autophagy was analysed by western blotting. β-actin was used as loading control. b Cells were treated with nimbolide for 6, 12 and 24 h. The LC-3 II puncta formation was viewed and captured by confocal microscope. LC-3 (green) and DAPI (nuclei, blue). Scalebar: 20 μm. c Oral cancer cells were treated with nimbolide for 0 to 96 h. Cell lysates were subjected to western blotting with Bax, Bcl-2, cytochrome c, cleaved caspase-9 and -3 antibodies. β-actin was used as loading control

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