Fig. 6: S1PR1/STAT3/VEGF-A signaling is critical for ERO1α-mediated promotion of migration, invasion, and angiogenesis. | Cell Death & Disease

Fig. 6: S1PR1/STAT3/VEGF-A signaling is critical for ERO1α-mediated promotion of migration, invasion, and angiogenesis.

From: Endoplasmic reticulum resident oxidase ERO1-Lalpha promotes hepatocellular carcinoma metastasis and angiogenesis through the S1PR1/STAT3/VEGF-A pathway

Fig. 6

a S1PR1 expression was analyzed in HCC tissues from the HCC cohort with or without metastasis described in Fig. 2. b Positive correlation between ERO1α and S1PR1 levels in HCC specimens. c Immunofluorescent staining images for ERO1α and S1PR1 in SMMC-7721-ERO1α and SMMC-7721-Vector cells. 4′,6-Diamidino-2-phenylindole (blue) was used to identify nuclei. Scale bars = 50 μm. d Indicated molecules were evaluated by western blots with four HCC cell lines, using GAPDH as the loading control. e RT-qPCR and f western blots for S1PR1 and VEGF-A in indicated cells. h–j Rescue experiments for ERO1α-overexpressing cells with S1PR1 silencing. Downregulated S1PR1 counteracted Huh-7 and SMMC-7721 cell migration and invasion that was enhanced by ERO1α overexpression. Cell migration and invasion were quantified as cell numbers. All experiments were performed three in triplicate. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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