Fig. 2: Loss of MOB1A/B leads to YAP activation and hyperplasia in the crypt and villus. | Cell Death & Disease

Fig. 2: Loss of MOB1A/B leads to YAP activation and hyperplasia in the crypt and villus.

From: Depletion of MOB1A/B causes intestinal epithelial degeneration by suppressing Wnt activity and activating BMP/TGF-β signaling

Fig. 2

a Western blot analysis of IECs isolated seven days after tamoxifen treatment in three individual control and MOB1A/B iKO mice. b Relative expression levels of Yap and Taz mRNA in 5 or 10 days after tamoxifen treatment in three individual control and MOB1A/B iKO mice. c Representative IHC staining of intestines with anti-YAP antibody at the indicated times after tamoxifen treatment. Scale bars, 20 μm. d Representative IHC staining of intestines with anti-Ki67 antibody at 4 days after tamoxifen treatment. Scale bars, 50 μm. e, f H&E staining (e) and TUNEL staining (f) of control and MOB1A/B-depleted intestines seven days after tamoxifen treatment. The upper regions of the yellow dotted lines indicate IECs. Scale bars, 200 μm (e) and 500 μm (f), respectively. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001

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