Fig. 3: Depletion of MOB1A/B induces changes in stem niche factors leading to degeneration of ISCs/crypt. | Cell Death & Disease

Fig. 3: Depletion of MOB1A/B induces changes in stem niche factors leading to degeneration of ISCs/crypt.

From: Depletion of MOB1A/B causes intestinal epithelial degeneration by suppressing Wnt activity and activating BMP/TGF-β signaling

Fig. 3

a Heatmap of IECs isolated from tamoxifen-treated mice (n = 2) for the indicated periods showing 13,700 downregulated genes and 5912 upregulated genes in rank order of fold change value at 7 days. Labeled genes are examples known to be involved in Wnt, Notch, EGF, TGF-β, and BMP signaling and ISCs markers. b Representative IHC staining with anti-CD44 antibody at the 7 days after tamoxifen treatment in three individual control and MOB1A/B-depleted mice. Scale bars, 20 μm. c Relative mRNA expression levels of ISCs marker genes (Lgr5, Olfm4, and Ascl2) in isolated IECs of control and MOB1A/B iKO mice (n = 3). d Representative bright-field images of intestinal organoids and the percentage of organoids showing 0–3 or ≥4 de novo crypt formation. MOB1A/B-depleted ISCs were isolated from control or tamoxifen-treated MOB1A/B iKO mice and analysis of crypt formation was performed 4 days after ISCs isolation (n = 3, n represents the number of separate cultures). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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