Fig. 6: Inhibition of TGF-β or BMP signaling restores defects in secretory lineage differentiation in MOB1A/B-depleted IECs. | Cell Death & Disease

Fig. 6: Inhibition of TGF-β or BMP signaling restores defects in secretory lineage differentiation in MOB1A/B-depleted IECs.

From: Depletion of MOB1A/B causes intestinal epithelial degeneration by suppressing Wnt activity and activating BMP/TGF-β signaling

Fig. 6

a Representative IHC staining with anti-CD44 antibodies in three individual control and MOB1A/B-depleted intestines sampled 7 days after treatment with tamoxifen and/or indicated inhibitors. The lower images are an enlarged view of red-dotted box in the upper images. Scale bars, 100 μm (upper) and 20 μm (lower). b Representative IHC staining with anti-CD44 antibodies and Alcian blue staining in three individual control and MOB1A/B-depleted intestines sampled 7 days after treatment with tamoxifen and/or indicated inhibitors. Scale bars, 20 μm. c Quantitative results showing expression levels of Atoh1 mRNA from IECs isolated from LDN- or SB-treated control and MOB1A/B iKO mice (n = 3). d Representative immunofluorescence image of intestines of indicated mice (n = 3). Dashed lines depict the crypt structure. Scale bars, 20 μm. e Quantitative results showing expression levels of Lgr5, Olfm4, and Ascl2 mRNA from IECs isolated from LDN- or SB-treated control and MOB1A/B iKO mice (n = 3). Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001

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