Fig. 2: YAP1 enhances proliferation by suppressing the clock | Cell Death & Disease

Fig. 2: YAP1 enhances proliferation by suppressing the clock

From: YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma

Fig. 2: YAP1 enhances proliferation by suppressing the clockThe alternative text for this image may have been generated using AI.

a Gene expression analysis of microarray performed on KP cells treated with 2 μm SAHA/0.5 μm JQ1 for 48 h. b qRT-PCR validation of clock genes in KP cells treated as in a. c Western blot of KP cells treated as in a. d qRT-PCR of human UPS cells (STS-109) treated as in a. e Western blot of STS-109 cells treated as in a. f (left) Bmal luciferase reporter assay in KP cells expressing Yap1 siRNA (20 nM) and treated with 2 μm SAHA/0.5 μm JQ1 for 12 h to activate signaling. (right) Western blot of YAP1 levels in siRNA treated cells. g Bmal luciferase reporter assay in KP cells treated with 2 μm SAHA/0.5 μm JQ1. h (left) Cell counting rescue proliferation assay in Yap1#1 and Arntl shRNA expressing KP cells. (right) qRT-PCR of Yap1 and Arntl expression in KP cells treated as in h. i Western blot of KP cells expressing multiple  Yap1 shRNAs and treated with 1 μm Staurosporin (12 h) as positive control. Error bars represent SD

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