Fig. 5: SAHA/JQ1 treatment promotes UPR target oscillation | Cell Death & Disease

Fig. 5: SAHA/JQ1 treatment promotes UPR target oscillation

From: YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma

Fig. 5: SAHA/JQ1 treatment promotes UPR target oscillationThe alternative text for this image may have been generated using AI.

a qRT-PCR of KP cells treated with 2 μm SAHA and/or 0.5 μm JQ1 for 0–120 h. b Summary graphs of qRT-PCR for TXNIP and DDIT3 treated as in a, b with the addition of 0–24 h time points. Some of the data summarized here are found in supplementary figure 5. c ATF4-GFP reporter assay in KP cells treated as in a for 24 h. Scale bar = 50 μm. d ATF6-GFP reporter assay in KP cells treated as in a for 24 h. Scale bar = 50 μm. e Scale bar = μm f Representative images of IHC from murine tumor sections from allograft experiments (shSCR, shRelA) and GEMMs (KP, KPY, and KP tumors treated with SAHA/JQ1 once daily with 25mg/kg SAHA and twice daily with 25 mg/kg JQ1). Tumors harvested after 20 days of treatment. Scale bar = 20 μm. g Quantification of Gadd34 expression from f. n = 3 mice per group, 12 images per tumor sample. Error bars represent SD

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