Fig. 6: TXNDC9 was trans-activated by FOXA1, JUND, and FOSL2 in HepG2. | Cell Death & Disease

Fig. 6: TXNDC9 was trans-activated by FOXA1, JUND, and FOSL2 in HepG2.

From: TXNDC9 promotes hepatocellular carcinoma progression by positive regulation of MYC-mediated transcriptional network

Fig. 6

a Overview of the loci of TXNDC9 in HepG2 cells. Refseq annotations are shown at the bottom. ChIP-seq datasets used in this analysis were retrieved from the NCBI Gene Expression Omnibus database. b FOXA1, JUND, and FOSL2 synergistically activated the promoter activity of TXNDC9. Dual luciferase reporter assays were used to evaluate the effects of FOXA1, JUND, and FOSL2 on the promoter activity of TXNDC9. Luciferase activity were normalized with cells transfected with pGL3-basic vector and pcDNA3.1 vector. c Knockdown FOXA1, JUND or FOSL2 decreased the expression of TXNDC9. Relative expression of TXNDC9 were detected in HepG2 cells transfected with FOXA1, JUND, FOSL2, or negative control (N.C.) siRNAs. Error bars represent the mean ± SD from three independent experiments. d Role of TXNDC9 in HCC. TXNDC9 was activated by FOXA1, JUND, and FOSL2 in HCC and overexpressed TXNDC9 promote the MYC-mediated transcriptional network and thus leads to cell proliferation of HCC

Back to article page