Fig. 2: UCA1 promotes cell migration and invasion of GC in vitro and in vivo.

a The effect of ectopic UCA1 expression in cell migration was assessed by transwell assay in BGC-823 and SGC-7901. The bar plot shows the mean number of migrating cells (±SD) per microscopic field from triplicate samples. b The effect of ectopic UCA1 expression in cell invasion was assessed by transwell assay in BGC-823 and SGC-7901. The bar plot shows the mean number of invading cells (±SD) per microscopic field from triplicate samples. c The effect of silencing UCA1 expression on cell migration was monitored by transwell assay in BGC-823 and SGC-7901. The bar plot shows the mean number of migrating cells (±SD) per microscopic field from triplicate samples. d The effect of silencing UCA1 expression on cell invasion was monitored by transwell assay in BGC-823 and SGC-7901. The bar plot shows the mean number of invading cells (±SD) per microscopic field from triplicate samples. e, f SGC-7901-Lv-UCA1 and BGC-Lv-shUCA1 stable cells were intravenously injected into BALB/c nude mice via tail vein. After 42 days, the livers were resected. g Detectable nodules were quantitatively evaluated on the surface of the livers. Data are expressed as the mean ± SD. (* p < 0.05; ** p < 0.01; one-way ANOVA). h Representative histological photomicrographs of liver tissue sections stained with H&E (scale bars = 50 μm and 100 μm). i The cell morphology of BGC-823 cells transfected with Lv-NC and Lv-UCA1 was observed by phase-contrast microscopy. j expression level of EMT-related markers was changed in Lv-UCA1 compared with control